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Novus Biologicals tgr5 antibodies

A The result of molecular docking between <t>TGR5</t> and butyric acid. B TGR5 luciferase activity was measured in NaB-treated HepG2 cells using a dual-luciferase reporter assay. C The protein expression of TGR5 in the liver of mice through IHC staining. Scale bars were 50 µm. D The relative protein expression of TGR5 in the liver of mice by Western blot. E The protein expression of TGR5 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. F The relative protein expression of TGR5 in NaB treated HepG2 cells by Western blot. G Effects of TGR5 knockdown on the levels of liver function indicators in HepG2 cells. H Effects of TGR5 knockdown on the levels of LDH in HepG2 cells. I Effects of TGR5 knockdown on the levels of IL-1β, IL-6 and TNF-α in the medium of HepG2 cells. J The relative protein expression of inflammatory factors in HepG2 cells by Western blot. K The protein expression of Collagen Ⅳ and Laminin in HepG2 cells knockdown TGR5 by IF staining. Scale bars were 20 µm. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m or HG + FFA; # P < 0.05, ## P < 0.01, compared to db/db or HG + FFA+NaB-H + TGR5-shRNA-Veh; % P < 0.05, %% P < 0.01, compared to NG; $ P < 0.05, compared to HG; && P < 0.01, compared to NG+NaB-H.

Tgr5 Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nbp2+23669/TGR5%2FGPBAR1+Antibody+-+BSA+Free/pmc12606137-368-0-7
Average 93 stars, based on 18 article reviews

tgr5 antibodies - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "Role of intestinal SCFAs homeostasis in the hepatoprotective effect of Clostridium butyricum in T2DM"

Article Title: Role of intestinal SCFAs homeostasis in the hepatoprotective effect of Clostridium butyricum in T2DM

Journal: NPJ Biofilms and Microbiomes

doi: 10.1038/s41522-025-00824-5

A The result of molecular docking between TGR5 and butyric acid. B TGR5 luciferase activity was measured in NaB-treated HepG2 cells using a dual-luciferase reporter assay. C The protein expression of TGR5 in the liver of mice through IHC staining. Scale bars were 50 µm. D The relative protein expression of TGR5 in the liver of mice by Western blot. E The protein expression of TGR5 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. F The relative protein expression of TGR5 in NaB treated HepG2 cells by Western blot. G Effects of TGR5 knockdown on the levels of liver function indicators in HepG2 cells. H Effects of TGR5 knockdown on the levels of LDH in HepG2 cells. I Effects of TGR5 knockdown on the levels of IL-1β, IL-6 and TNF-α in the medium of HepG2 cells. J The relative protein expression of inflammatory factors in HepG2 cells by Western blot. K The protein expression of Collagen Ⅳ and Laminin in HepG2 cells knockdown TGR5 by IF staining. Scale bars were 20 µm. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m or HG + FFA; # P < 0.05, ## P < 0.01, compared to db/db or HG + FFA+NaB-H + TGR5-shRNA-Veh; % P < 0.05, %% P < 0.01, compared to NG; $ P < 0.05, compared to HG; && P < 0.01, compared to NG+NaB-H.

Figure Legend Snippet: A The result of molecular docking between TGR5 and butyric acid. B TGR5 luciferase activity was measured in NaB-treated HepG2 cells using a dual-luciferase reporter assay. C The protein expression of TGR5 in the liver of mice through IHC staining. Scale bars were 50 µm. D The relative protein expression of TGR5 in the liver of mice by Western blot. E The protein expression of TGR5 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. F The relative protein expression of TGR5 in NaB treated HepG2 cells by Western blot. G Effects of TGR5 knockdown on the levels of liver function indicators in HepG2 cells. H Effects of TGR5 knockdown on the levels of LDH in HepG2 cells. I Effects of TGR5 knockdown on the levels of IL-1β, IL-6 and TNF-α in the medium of HepG2 cells. J The relative protein expression of inflammatory factors in HepG2 cells by Western blot. K The protein expression of Collagen Ⅳ and Laminin in HepG2 cells knockdown TGR5 by IF staining. Scale bars were 20 µm. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m or HG + FFA; # P < 0.05, ## P < 0.01, compared to db/db or HG + FFA+NaB-H + TGR5-shRNA-Veh; % P < 0.05, %% P < 0.01, compared to NG; $ P < 0.05, compared to HG; && P < 0.01, compared to NG+NaB-H.

Techniques Used: Luciferase, Activity Assay, Reporter Assay, Expressing, Immunohistochemistry, Western Blot, Staining, Knockdown, shRNA

A Safety of HepG2 cells in different concentrations of SBI-115. B The protein expression of TGR5 in HepG2 cells by IF staining. Scale bars were 20 µm. C The levels of liver function indicators in HepG2 cells. D The levels of LDH in HepG2 cells treated with SBI-115. E The levels of IL-1β, IL-6 and TNF-α in HepG2 cells treated with SBI-115. F The relative protein expression of inflammatory factors in SBI-115 treated HepG2 cells by Western blot. G The protein expression of Collagen Ⅳ and Laminin in SBI-115 treated HepG2 cells by IF staining. Scale bars were 20 µm. Each bar represents the mean ± SEM for cell groups of three. ** P < 0.01, compared to HG + FFA; & P < 0.05, && P < 0.01, compared to HG + FFA+NaB-H.

Figure Legend Snippet: A Safety of HepG2 cells in different concentrations of SBI-115. B The protein expression of TGR5 in HepG2 cells by IF staining. Scale bars were 20 µm. C The levels of liver function indicators in HepG2 cells. D The levels of LDH in HepG2 cells treated with SBI-115. E The levels of IL-1β, IL-6 and TNF-α in HepG2 cells treated with SBI-115. F The relative protein expression of inflammatory factors in SBI-115 treated HepG2 cells by Western blot. G The protein expression of Collagen Ⅳ and Laminin in SBI-115 treated HepG2 cells by IF staining. Scale bars were 20 µm. Each bar represents the mean ± SEM for cell groups of three. ** P < 0.01, compared to HG + FFA; & P < 0.05, && P < 0.01, compared to HG + FFA+NaB-H.

Techniques Used: Expressing, Staining, Western Blot

A The relative phosphorylation levels of NF-κB in the liver of mice by IF staining. Scale bars were 50 µm (above) and 20 µm (below). B The relative phosphorylation levels of NF-κB-p65 in the liver of NaB treated mice by Western Blot. C The relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. D The relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by Western Blot. E Effects of TGR5 knockdown on the relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. F Effects of TGR5 knockdown on the relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by Western Blot. G Luciferse activity of NF-κB promoter in HepG2 cells. H EMSA of TGR5 blinding to the NF-κB promoter in HepG2 cells. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m or NG; ## P < 0.01, compared to db/db or HG; $ P < 0.05, $$ P < 0.01, compared to HG + FFA; % P < 0.05, %% P < 0.01, compared to HG + FFA + NaB + TGR5-shRNA-Veh.

Figure Legend Snippet: A The relative phosphorylation levels of NF-κB in the liver of mice by IF staining. Scale bars were 50 µm (above) and 20 µm (below). B The relative phosphorylation levels of NF-κB-p65 in the liver of NaB treated mice by Western Blot. C The relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. D The relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by Western Blot. E Effects of TGR5 knockdown on the relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. F Effects of TGR5 knockdown on the relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by Western Blot. G Luciferse activity of NF-κB promoter in HepG2 cells. H EMSA of TGR5 blinding to the NF-κB promoter in HepG2 cells. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m or NG; ## P < 0.01, compared to db/db or HG; $ P < 0.05, $$ P < 0.01, compared to HG + FFA; % P < 0.05, %% P < 0.01, compared to HG + FFA + NaB + TGR5-shRNA-Veh.

Techniques Used: Phospho-proteomics, Staining, Western Blot, Knockdown, Activity Assay, shRNA

A The phosphorylation levels of IκB-α in mice’s liver through IHC staining. Scale bars were 50 µm. B The relative phosphorylation levels of IκB-α in mice’s liver by Western Blot. C The protein levels of β-arrestin2 in mice’s liver by IF staining. Scale bars were 50 µm (above) and 20 µm (below). D The relative phosphorylation levels of IκB-α in NaB treated HepG2 cells by Western Blot. E Effects of NaB on the levels of β-arrestin2 in HepG2 cells by IF staining. Scale bars were 20 µm. F Effects of TGR5 knockdown on the relative phosphorylation levels of IκB-α in NaB treated HepG2 cells by Western Blot. G Effects of TGR5 inhibitor on the relative phosphorylation levels of IκB-α in NaB treated HepG2 cells by Western Blot. H Effects of TGR5 knockdown on the relative protein expression of β-arrestin2 (green) and IκB-α (red) in NaB treated HepG2 cells by IF double staining. Scale bars were 20 µm. I Effects of TGR5 inhibitor on the relative protein levels of β-arrestin2 (green) and IκB-α (red) in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. J The interactions between β-arrestin2 and IκB-α were detected by Co-IP in TGR5 knockdown HepG2 cells. K The interactions between β-arrestin2 and IκB-α were detected by Co-IP in SBI-115 treated HepG2 cells. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m mice or NG as indicated. ## P < 0.01, compared to db/db mice or HG; %% P < 0.01, compared to HG + FFA+NaB; $$ P < 0.01, compared to HG + FFA; && P < 0.01, compared to HG + FFA+NaB-H + TGR5-shRNA-Veh.

Figure Legend Snippet: A The phosphorylation levels of IκB-α in mice’s liver through IHC staining. Scale bars were 50 µm. B The relative phosphorylation levels of IκB-α in mice’s liver by Western Blot. C The protein levels of β-arrestin2 in mice’s liver by IF staining. Scale bars were 50 µm (above) and 20 µm (below). D The relative phosphorylation levels of IκB-α in NaB treated HepG2 cells by Western Blot. E Effects of NaB on the levels of β-arrestin2 in HepG2 cells by IF staining. Scale bars were 20 µm. F Effects of TGR5 knockdown on the relative phosphorylation levels of IκB-α in NaB treated HepG2 cells by Western Blot. G Effects of TGR5 inhibitor on the relative phosphorylation levels of IκB-α in NaB treated HepG2 cells by Western Blot. H Effects of TGR5 knockdown on the relative protein expression of β-arrestin2 (green) and IκB-α (red) in NaB treated HepG2 cells by IF double staining. Scale bars were 20 µm. I Effects of TGR5 inhibitor on the relative protein levels of β-arrestin2 (green) and IκB-α (red) in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. J The interactions between β-arrestin2 and IκB-α were detected by Co-IP in TGR5 knockdown HepG2 cells. K The interactions between β-arrestin2 and IκB-α were detected by Co-IP in SBI-115 treated HepG2 cells. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m mice or NG as indicated. ## P < 0.01, compared to db/db mice or HG; %% P < 0.01, compared to HG + FFA+NaB; $$ P < 0.01, compared to HG + FFA; && P < 0.01, compared to HG + FFA+NaB-H + TGR5-shRNA-Veh.

Techniques Used: Phospho-proteomics, Immunohistochemistry, Western Blot, Staining, Knockdown, Expressing, Double Staining, Co-Immunoprecipitation Assay, shRNA

Schematic diagram depicting the possible mechanism of CB against hepatitis in T2DM through activating hepatic TGR5 via intestinal butyric acid, leading to the inactivated IκB-α/β-arrestin2/NF-κB signaling pathway.

Figure Legend Snippet: Schematic diagram depicting the possible mechanism of CB against hepatitis in T2DM through activating hepatic TGR5 via intestinal butyric acid, leading to the inactivated IκB-α/β-arrestin2/NF-κB signaling pathway.

Techniques Used:

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Article Title: Activation of GPBAR1 attenuates vascular inflammation and atherosclerosis in a mouse model of NAFLD-related cardiovascular disease.
Article Snippet: .. Primary antibodies, anti-GPBAR-1 (1:100) (NBP2-23669, Novus Biologicals, Littleton, CO), anti-α-SMA (1:500) (14–9760-82, eBioscience, San Diego, CA), anti-PECAM-1 (1:100) (BONDTM Ready-toUse Primary Antibody CD31, JC70A) and anti-CD11b (1:50) (M1/70.15 Novus biologicals, Littleton, CO) were incubated overnight at 4 ◦C. .. The next day, after 3 washes with PBS 1X containing 0.1 % Tween 20 (PBST), sections were incubated with secondary antibody Goat antiRabbit IgG (H + L) and Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (a11011, Invitrogen, Carlsbad, CA) (1: 1000) for GPBAR1, with Goat anti-Mouse IgG (H + L) Highly Cross-adsorbed Secondary Antibody Alexa Fluor Plus 488 (a32723, Invitrogen, Carlsbad, CA) (1:200) for α-SMA, PECAM-1 and CD11b, and with Goat anti-Rat (1:50) (JA112-545–062, Jackson Immuno Research Pennsylvania, USA) for 1 h at room temperature in the dark.

Article Title: Defective Bile Acid Signaling Promotes Vascular Dysfunction, Supporting a Role for G‐Protein Bile Acid Receptor 1/Farnesoid X Receptor Agonism and Statins in the Treatment of Nonalcoholic Fatty Liver Disease
Article Snippet: .. Primary antibodies, anti-GPBAR-1 (1:100) (NBP2-23669, Novus Biologicals), anti-FXR (1:100) (ORB156973, Biorbyt) and anti-α-SMA (1:500) (14-9760-82, eBioscience Invitrogen), anti-PECAM-1 (1:100) (BONDTM Ready-to-Use Primary Antibody CD31, JC70A) and anti-CD11b (1:50) (M1/70.15 Novus biologicals) and were incubated overnight at 4°. .. The next day, after 3 washes with PBS 1X containing 0,1 % Tween 20 (PBST), sections were incubated with secondary antibody Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 (a11011, Invitrogen, Thermofisher scientific) (1: 1000) for GPBAR1 and FXR, with Goat anti-Mouse IgG (H + L) Highly Cross-adsorbed Secondary Antibody Alexa Fluor Plus 488 (a32723, Invitrogen) (1:200) for α-SMA and PECAM-1, and with Goat anti-Rat (1:50) (JA112-545-062, Jackson Immuno Research) for 1h at room temperature in the dark.

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Journal: NPJ Biofilms and Microbiomes

Article Title: Role of intestinal SCFAs homeostasis in the hepatoprotective effect of Clostridium butyricum in T2DM

doi: 10.1038/s41522-025-00824-5

Figure Lengend Snippet: A The result of molecular docking between TGR5 and butyric acid. B TGR5 luciferase activity was measured in NaB-treated HepG2 cells using a dual-luciferase reporter assay. C The protein expression of TGR5 in the liver of mice through IHC staining. Scale bars were 50 µm. D The relative protein expression of TGR5 in the liver of mice by Western blot. E The protein expression of TGR5 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. F The relative protein expression of TGR5 in NaB treated HepG2 cells by Western blot. G Effects of TGR5 knockdown on the levels of liver function indicators in HepG2 cells. H Effects of TGR5 knockdown on the levels of LDH in HepG2 cells. I Effects of TGR5 knockdown on the levels of IL-1β, IL-6 and TNF-α in the medium of HepG2 cells. J The relative protein expression of inflammatory factors in HepG2 cells by Western blot. K The protein expression of Collagen Ⅳ and Laminin in HepG2 cells knockdown TGR5 by IF staining. Scale bars were 20 µm. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m or HG + FFA; # P < 0.05, ## P < 0.01, compared to db/db or HG + FFA+NaB-H + TGR5-shRNA-Veh; % P < 0.05, %% P < 0.01, compared to NG; $ P < 0.05, compared to HG; && P < 0.01, compared to NG+NaB-H.

Article Snippet: TGR5 antibodies (NBP2-23669SS, ab72608) were purchased from Novus Biologicals (CO, USA) or Abcam (CA, USA).

Techniques: Luciferase, Activity Assay, Reporter Assay, Expressing, Immunohistochemistry, Western Blot, Staining, Knockdown, shRNA

Journal: NPJ Biofilms and Microbiomes

Article Title: Role of intestinal SCFAs homeostasis in the hepatoprotective effect of Clostridium butyricum in T2DM

doi: 10.1038/s41522-025-00824-5

Figure Lengend Snippet: A Safety of HepG2 cells in different concentrations of SBI-115. B The protein expression of TGR5 in HepG2 cells by IF staining. Scale bars were 20 µm. C The levels of liver function indicators in HepG2 cells. D The levels of LDH in HepG2 cells treated with SBI-115. E The levels of IL-1β, IL-6 and TNF-α in HepG2 cells treated with SBI-115. F The relative protein expression of inflammatory factors in SBI-115 treated HepG2 cells by Western blot. G The protein expression of Collagen Ⅳ and Laminin in SBI-115 treated HepG2 cells by IF staining. Scale bars were 20 µm. Each bar represents the mean ± SEM for cell groups of three. ** P < 0.01, compared to HG + FFA; & P < 0.05, && P < 0.01, compared to HG + FFA+NaB-H.

Article Snippet: TGR5 antibodies (NBP2-23669SS, ab72608) were purchased from Novus Biologicals (CO, USA) or Abcam (CA, USA).

Techniques: Expressing, Staining, Western Blot

Journal: NPJ Biofilms and Microbiomes

Article Title: Role of intestinal SCFAs homeostasis in the hepatoprotective effect of Clostridium butyricum in T2DM

doi: 10.1038/s41522-025-00824-5

Figure Lengend Snippet: A The relative phosphorylation levels of NF-κB in the liver of mice by IF staining. Scale bars were 50 µm (above) and 20 µm (below). B The relative phosphorylation levels of NF-κB-p65 in the liver of NaB treated mice by Western Blot. C The relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. D The relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by Western Blot. E Effects of TGR5 knockdown on the relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. F Effects of TGR5 knockdown on the relative phosphorylation levels of NF-κB-p65 in NaB treated HepG2 cells by Western Blot. G Luciferse activity of NF-κB promoter in HepG2 cells. H EMSA of TGR5 blinding to the NF-κB promoter in HepG2 cells. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m or NG; ## P < 0.01, compared to db/db or HG; $ P < 0.05, $$ P < 0.01, compared to HG + FFA; % P < 0.05, %% P < 0.01, compared to HG + FFA + NaB + TGR5-shRNA-Veh.

Article Snippet: TGR5 antibodies (NBP2-23669SS, ab72608) were purchased from Novus Biologicals (CO, USA) or Abcam (CA, USA).

Techniques: Phospho-proteomics, Staining, Western Blot, Knockdown, Activity Assay, shRNA

Journal: NPJ Biofilms and Microbiomes

Article Title: Role of intestinal SCFAs homeostasis in the hepatoprotective effect of Clostridium butyricum in T2DM

doi: 10.1038/s41522-025-00824-5

Figure Lengend Snippet: A The phosphorylation levels of IκB-α in mice’s liver through IHC staining. Scale bars were 50 µm. B The relative phosphorylation levels of IκB-α in mice’s liver by Western Blot. C The protein levels of β-arrestin2 in mice’s liver by IF staining. Scale bars were 50 µm (above) and 20 µm (below). D The relative phosphorylation levels of IκB-α in NaB treated HepG2 cells by Western Blot. E Effects of NaB on the levels of β-arrestin2 in HepG2 cells by IF staining. Scale bars were 20 µm. F Effects of TGR5 knockdown on the relative phosphorylation levels of IκB-α in NaB treated HepG2 cells by Western Blot. G Effects of TGR5 inhibitor on the relative phosphorylation levels of IκB-α in NaB treated HepG2 cells by Western Blot. H Effects of TGR5 knockdown on the relative protein expression of β-arrestin2 (green) and IκB-α (red) in NaB treated HepG2 cells by IF double staining. Scale bars were 20 µm. I Effects of TGR5 inhibitor on the relative protein levels of β-arrestin2 (green) and IκB-α (red) in NaB treated HepG2 cells by IF staining. Scale bars were 20 µm. J The interactions between β-arrestin2 and IκB-α were detected by Co-IP in TGR5 knockdown HepG2 cells. K The interactions between β-arrestin2 and IκB-α were detected by Co-IP in SBI-115 treated HepG2 cells. Each bar represents the mean ± SEM for mice groups of six and cell groups of three. ** P < 0.01, compared to db/m mice or NG as indicated. ## P < 0.01, compared to db/db mice or HG; %% P < 0.01, compared to HG + FFA+NaB; $$ P < 0.01, compared to HG + FFA; && P < 0.01, compared to HG + FFA+NaB-H + TGR5-shRNA-Veh.

Article Snippet: TGR5 antibodies (NBP2-23669SS, ab72608) were purchased from Novus Biologicals (CO, USA) or Abcam (CA, USA).

Techniques: Phospho-proteomics, Immunohistochemistry, Western Blot, Staining, Knockdown, Expressing, Double Staining, Co-Immunoprecipitation Assay, shRNA

Journal: NPJ Biofilms and Microbiomes

Article Title: Role of intestinal SCFAs homeostasis in the hepatoprotective effect of Clostridium butyricum in T2DM

doi: 10.1038/s41522-025-00824-5

Figure Lengend Snippet: Schematic diagram depicting the possible mechanism of CB against hepatitis in T2DM through activating hepatic TGR5 via intestinal butyric acid, leading to the inactivated IκB-α/β-arrestin2/NF-κB signaling pathway.

Article Snippet: TGR5 antibodies (NBP2-23669SS, ab72608) were purchased from Novus Biologicals (CO, USA) or Abcam (CA, USA).

Techniques:

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tgr5 antibodies (Novus Biologicals)

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